The unnamed man, believed to be in his late 20s, told police he was sexually molested after the male driver made a detour to supposedly pick up someone else.
A source close to the investigation said: ‘The driver suddenly stopped and one of the women quickly covered his eyes while the others held his feet and hands together.
‘The man said they forced him to drink a substance from a bottle and he passed out.
‘He suspects they took turns to sexually assault him and took away his semen.
‘After waking up he put on his clothes left at the scene and walked to the main road where he got a lift to the police station.’
Officers probing the case in Lupane, Zimbabwe, are treating it as aggravated indecent assault.
The man, who was taken to St. Luke’s Hospital, said he was offered a lift to Bulawayo in a Toyota Quantum with South Africa number plates.
So-called ‘semen harvesters’ first made headlines in 2011 when they pounced on motorists on a road between Gweru and Harare.
Three women were arrested with 31 condoms full of semen after they were caught at a roadblock.
And last year three other women reportedly abducted a man in Bulawayo and forced him to have sex with them before vanishing with his semen.
Inspector Eglon Nkala appealed for witness in a bid to crack the latest case.